C7488
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 | Immunoblot Whole cell extract of A431 cells treated with nocodazole was separated on SDS-PAGE and blotted with Rabbit Anti-CENP-E (Cat. No. C7488). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes A. Antibody dilution 1:2,000 B. Antibody dilution 1:2,000 + CENP-E immunizing peptide (10 μg/mL) |
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C7488
Sigma
Anti-CENP-E antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | Anti-Centromere-associated protein-E |
| MDL number: | MFCD05665206 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 2545-2563 located near the C-terminus of human CENP-E conjugated to KLH. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~310 kDa |
| species reactivity | human |
| application(s) | immunoblotting: 1:2,000 using whole extract of HeLa nuclear cell line |
| immunocytochemistry: suitable using human epitheloid carcinoma HeLa cell line. | |
| microarray: suitable | |
| Gene Information | human ... CENPE(1062) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
